Looking for:
Molecular cloning a laboratory manual 4th edition pdf free download.Molecular Cloning A Laboratory Manual Volume 1, 2, & 3 PDF |
- Download Molecular Cloning A Laboratory Manual PDF | Free PDF Books
To browse Academia. Skip to main content. By using our site, you agree to our collection of information through the use of cookies. To learn more, view our Privacy Policy. Log In Sign Up. Download Free PDF. Click here for more information or to buy the book. Rizkiani Nurro Download PDF. A short summary of this paper.
Green, Joseph Sambrook. Includes bibliographical references and index. Molecular cloning—Laboratory manuals. Sambrook, Joseph. Molecular cloning. S26 Cold Spring Harbor Laboratory makes no repre- sentations or warranties with respect to the material set forth in this manual and has no liability in connection with the use of these materials.
All registered trademarks, trade names, and brand names mentioned in this book are the property of the respective owners. Readers should please consult individual manufacturers and other resources for current and specific product information. With the exception of those suppliers listed in the text with their addresses, all suppliers mentioned in this manual can be found on the BioSupplyNet Web site at www.
All World Wide Web addresses are accurate to the best of our knowledge at the time of printing. Procedures for the humane treatment of animals must be observed at all times. Check with the local animal facility for guidelines. Certain experimental procedures in this manual may be the subject of national or local legislation or agency restrictions.
Users of this manual are responsible for obtaining the relevant permissions, certificates, or licenses in these cases. Neither the authors of this manual nor Cold Spring Harbor Laboratory assume any responsibility for failure of a user to do so. The materials and methods in this manual may infringe the patent and proprietary rights of other individuals, companies, or organiza- tions.
Users of this manual are responsible for obtaining any licenses necessary to use such materials and to practice such methods. Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Cold Spring Harbor Laboratory Press, provided that the appropriate fee is paid directly to the Copyright Clearance Center CCC.
Prior to photocopying items for educational classroom use, contact CCC at the above address. Reece-Hoyes and Albertha J. Kielkopf, William Bauer, and Ina L. Exploration of the vast genomic landscape required the development of a great variety of new exper- imental techniques and approaches. Inevitably, venerable cloning manuals became outdated and established methods were rendered obsolete.
These events provided the major impetus for a thor- ough revision of Molecular Cloning. In the first stages of Molecular Cloning 4 MC4 , we undertook an extensive review process to determine what old material should be kept, what new material should be added, and, most difficult of all, what material should be deleted. The names of the many scientists who contributed invaluable advice during the review process are listed on the following acknowledgments page.
We are grateful to them all. It is of course impossible to include in any single laboratory manual all experimental methods used in molecular biology, and so choices, sometimes tough ones, had to be made. Individuals may disagree with some of our choices and we suspect some will. However, our two guiding principles are as follows.
So, although MC4 contains a chapter on yeast two-hybrid procedures for analyzing protein—protein interactions, we did not include the many other approaches for studying protein—protein interactions that do not directly involve nucleic acids. However, our task was made somewhat easier by the agreement of our publisher, Cold Spring Harbor Laboratory Press, to make these older methods freely available on the Cold Spring Harbor Protocols website www. The explosion of new experimental approaches has made it impractical, if not impossible, for any single person or even two to write with authority on all the relevant experimental methods.
As a result, a major departure from previous editions of Molecular Cloning was the commissioning of experts in the field to write specific chapters and to contribute specific protocols. Without the enthusiastic participation of these scientists, MC4 simply would not exist. On the one hand, kits offer tre- mendous convenience, particularly for procedures that are not routinely used in an individual laboratory. On the other hand, kits can often be too convenient, enabling users to perform proce- dures without understanding the underlying principles of the method.
Where possible, we have attempted to deal with this dilemma by providing lists of commercially available kits and also describing how they work. There are many people who played essential roles in the production of MC4 and whom we gratefully acknowledge.
Ann Boyle was instrumental in getting MC4 off the ground and played a critical organizational role during the early stages of the project. Subsequently, her responsibilities were taken over by the able assistance of Alex Gann. Monica Salani made substantial contributions to the content and writing of Chapter 9.
Acknowledgments The authors wish to thank the following colleagues for their valuable assistance: H. General Safety and Hazardous Material Information This manual should be used by laboratory personnel with experience in laboratory and chemical safety or students under the supervision of suitably trained personnel.
The procedures, chemicals, and equipment referenced in this manual are hazardous and can cause serious injury unless per- formed, handled, and used with care and in a manner consistent with safe laboratory practices.
Students and researchers using the procedures in this manual do so at their own risk. All registered trademarks, trade names, and brand names mentioned in this book are the prop- erty of the respective owners. Readers should consult individual manufacturers and other resour- ces for current and specific product information. Appropriate sources for obtaining safety information and general guidelines for laboratory safety are provided in the General Safety and Hazardous Material appendix of this manual.
Related Papers. Methods in molecular biology. By avadh shah. By isabel silva. By Sylvia Vasiliou. YAC Protocols. By Jose Velazco. Human Molecular Genetics. By thuha vu. Download pdf. Remember me on this computer. Enter the email address you signed up with and we'll email you a reset link.
Need an account? Click here to sign up.
No comments:
Post a Comment